箭叶淫羊藿对去卵巢大鼠骨量丢失的保护作用

Prevention of bone loss by aqueous extract of Epimedii sagittatum in an ovariectomized rat model of osteoporosis

目的研究箭叶淫羊藿对去卵巢大鼠骨量丢失的保护作用。方法大鼠分去卵巢组和假手术组。去卵巢组包括空白对照组、雌激素组、0.5g/kg淫羊藿提取物组和1g/kg淫羊藿提取物组。11周后,测定大鼠血清钙、磷的浓度和碱性磷酸酶的活性,竞争放射免疫法测定血清中骨钙素、降钙素及雌二醇的浓度;双能X线吸收测量法测定股骨干骺端的骨密度。不脱钙切片法测定胫骨近端骨形态学参数。结果箭叶淫羊藿能显著提高去卵巢大鼠血清碱性磷酸酶活性,对体质量变化无明显影响,同时,箭叶淫羊藿能抑制去卵巢引起的子宫萎缩和骨密度的减少,还可以增加血清钙和雌二醇的浓度。组织形态学结果也显示箭叶淫羊藿可以减少去卵巢大鼠的骨小梁分离度,对骨小梁厚度和骨小梁数目无明显影响,而且可以显著提高骨形成率。结论箭叶淫羊藿水提物对去卵巢大鼠骨质疏松症具有防治作用。

Objective： To investigate the prevention effect of aqueous extract of Epimedii sagittatum （ESE） on ovariectomy-induced （OVX） bone loss in rats. Methods： Rats were divided into sham-operated and OVX groups. The OVX rats were divided into four groups treated with distilled water, 17β-estradiol （1 mg/kg, ig） and ESE （0.5 and 1 g /kg, ig） for 11 weeks. Serum calcium, phosphorus, estradiol, bone gla protein concentrations and serum alkaline phosphatase activity were measured. Bone density was assayed by dual-energy X-ray absorptiometry. The undecalcified longitudinal proximal tibial metaphysical sections were cut and stained for the bone histomorphometric analysis. Results： In OVX rats, alkaline phosphatase activity in serum was markedly increased by ESE treatment, which had no obvious influence on the body weight. Meanwhile, atrophy of uterus and descent of bone mineral density were suppressed by ESE treatment. In addition, ESE completely corrected the decreased concentrations of calcium and E2 in serum observed in OVX rats. Histological results also showed ESE prevented the increases in trabecular separation （Tb. Sp） in OVX rats whereas it did not alter trabecular thickness （Tb. Th） and trabecular number （Tb. N） in OVX rats. Moreover, ESE had remarkable effect on bone formation rate with bone volume as referent （BFR/ BV） and bone formation rate with bone surface as referent （BFR/BS）. Conclusion： The findings assessed on the basis of biochemical test, bone mineral density and histomorphometric parameters show that aqueous extract of Epimedii sagittatum has a definite antiosteoporotic effect and can prevent the OVX-induced bone loss in rats.