硝酸甘油耐受与氧化应激和3,4,5,6-四羟基酮的治疗作用

Oxidative stress in nitroglycerin tolerance and treatment with 3,4,5,6-tetrahydroxyxanthone

目的:探讨氧化应激与NTG耐受的关系及3,4,5,6-四羟基口山酮的治疗作用。方法:(1)在大鼠离体胸主动脉环检测NTG诱导的血管舒张反应。(2)培养内皮细胞,测定培养液中活性氧(radi-caloxygenspecies,ROS)水平及细胞内cGMP水平变化。结果:(1)3,4,5,6-四羟基口山酮可显著恢复NTG耐受血管对NTG的舒血管效应。(2)3,4,5,6-四羟基口山酮能显著抑制NTG诱导的细胞内ROS水平升高,3,4,5,6-四羟基口山酮能显著恢复NTG耐受内皮细胞的cGMP水平。结论:NTG耐受与氧化应激有关,3,4,5,6-四羟基口山酮有逆转NTG耐受作用,其作用与其抗氧化作用有关。

Objective To determine the relationship between the nitro the stimulation of radical oxygen species （ROS） production, and the therapeutic ycerin tolerance and effect of 3,4,5,6 - tetrahydroxyxanthone. Methods Vasodilator responses to nitroglycerin were examined in the isolated thoracic aorta. The contents of ROS, and cGMP were determined in the cultured human umbilical vein endothelial cells. Results 3,4,5,6-tetrahydroxyxanthone could significantly reduce the inhibition of relaxation by nitroglycerin. 3,4,5,6-tetrahydroxyxanthone could significantly inhibit the ROS increase and increase the cGMP level. Conclusion Nitroglycerin tolerance is associated with the stimulation of ROS production, and the reversal of nitroglycerin tolerance with 3,4,5,6-tetrahydroxyxanthone is related to the reduction of ROS.