摘要
目的:观察黄花倒水莲皂苷C抑制氧化型低密度脂蛋白(oxidizedlowdensitylipopprotein,ox-LDL)诱导的人脐静脉内皮细胞血凝素样氧化型低密度脂蛋白受体(oxidizedlowdesitylipoproteinrecep-tor-1,LOX-1)表达的作用。方法:培养人脐静脉内皮细胞株(HUVEC-12),随机分为:对照组、ox-LDL(50mg/L)组和氧化型低密度脂蛋白(50mg/L)+黄花倒水莲皂苷C(1,3,10μmol/L)组,通过RT-PCR和Westernblot蛋白印迹分析检测LOX-1mRNA和蛋白的表达。结果:ox-LDL上调LOX-1mRNA和蛋白的表达,黄花倒水莲皂苷C明显抑制ox-LDL诱导的LOX-1mRNA和蛋白的表达,且呈浓度依赖性。结论:黄花倒水莲皂苷C明显抑制ox-LDL诱导的内皮细胞LOX-1mRNA和蛋白的表达。
Objective To investigate the effect of reinioside C （ RC ） on the expression of lectin-like oxidized low density lipoprotein receptor （LOX）-1 mRNA and LOX-1 protein induced by ox- idized low density lipoprotein （ox-LDL） in cultured human umbilical vein endothelial cells （HUVEC ）. Methods presence of RC （ were examined by icantly raised the ent. Conclusion duced by ox-LDL HUVECs were cultured with ox-LDL （50 mg/L） for 24 h in the absence or 1, 3, and 10 μmol/L）. The expressions of LOX-1 mRNA and LOX-1 protein RT-PCR and Western-blot. Results Incubation with ox-LDL （50 mg/L） signifexpression of LOX-1 mRNA and LOX-1 protein, which was concentration-dependent.Conclusion RC can inhibit the increased expression of LOX-1 mRNA and LOX-1 protein induced by ox-LDL in HUVECs.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期659-662,共4页
Journal of Central South University ：Medical Science
