P21在单侧输尿管梗阻大鼠肾间质纤维化中的表达及依那普利对其表达的调节

P21 expression in renal interstitial fibrosis and regulative effect of enalapril

目的:动态观察P21在单侧输尿管梗阻肾间质纤维化大鼠模型中的表达,以及依那普利对其表达的调节。方法:将SD大鼠随机分为假手术组(SOR组)、单侧输尿管结扎组(UUO组)和依那普利治疗组(EnT组)。用免疫组织化学的方法检测3组大鼠术后7,14和21d肾小管上皮细胞P21的表达;用逆转录-聚合酶链式反应(RT-PCR)的方法检测肾脏p21mRNA的变化;并观察依那普利对肾脏P21表达的调节。结果:在UUO大鼠模型早期,肾小管上皮细胞P21以及肾脏p21mRNA的表达即有明显增高,随着梗阻时间的延长,P21的表达逐渐增强;依那普利能明显抑制P21的表达。结论:P21在UUO大鼠肾小管上皮细胞高表达,依那普利可抑制其表达。P21可能参与肾小管间质纤维化的发病过程。

Objective To investigate the expression of P21 in renal interstitial fibrosis rats and the effect of enalapril on it. Methods Sprague Dawley rats were randomly divided into 3 groups： a sham operation group, a unilateral urethral obstruction group, and an enalapril treatment group. The expression of P21 in renal tubular epithelial cells on the process was detected by immunohistochemistry at different time spots （7, 14, 21 d after UUO, sham-surgery or enalapril treatment）. The expression of p21 mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results Seven days after the surgery, significant differences were found in P21 expression between UUO and SOR renal tubular cells. With degree of interstitial fibrosis aggravating, P21 expression increased. Enalapril can inhibit its expression. Conclusion In the kidney of UUO rats, P21 expression increased and enalapril possessed significant inhibitory effects on the procedure. P21 may participate in the pathogenesis of renal tubule-interstitial fibrosis.