白松片对大鼠慢性应激抑郁模型的抗抑郁实验研究

Anti-depressive effect of Baisong tablets on the chronic mild unpredicted stress depression in rats

目的:探讨慢性应激抑郁模型大鼠神经生化、神经内分泌的变化及白松片对其的干预作用。方法:60只SD雄性大鼠随机分为正常对照组(NC组)、模型对照组(MC组)、氟西汀对照组(FC组)和白松片治疗组(BST),每组15只。采用长期轻度不可预见性应激+孤养复制大鼠抑郁模型,用高效液相色谱仪法检测海马5-羟色胺(5-HT)、谷氨酸(Glu)、γ-氨基丁酸(GABA)含量;放射免疫方法测定大鼠血浆促肾上腺皮质素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质醇(CORT)的水平;应用逆转录聚合酶联反应方法测定大鼠下丘脑和大脑皮质CRHmRNA表达水平;免疫组织化学法测定海马脑源性神经营养因子、酪氨酸羟化酶的表达。结果:与NC比较,模型大鼠海马5-HT,GABA含量显著降低;血浆CRH,ACTH,CORT及海马Glu含量增加;脑内CRHmRNA表达显著升高(P<0.01);与MC组比,BST组和FC组大鼠海马5-HT,GABA含量升高(P<0.01,或P<0.05);血浆CRH,ACTH,CORT及海马Glu含量降低;脑内CRHmRNA表达下降(P<0.01)。结论:慢性轻度不可预见性应激可使大鼠神经内分泌、神经生化发生异常改变,白松片对此具有一定调节作用。

Objective To investigate the effect of Baisong tablets on the nerv-biochemistry and neuroendocrine of chronic mild unpredicted stress depression in rats. Methods Sixty male Sprague-Dawley rats were randomly allocated to 4 groups： a normal control （NC） , a model control （ MC ） , a fluoxetine control （ FC ） and a Baisong tablet treatment group （ BST ）. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressor. The levels of serotonin （5-HT）, glutamate （Glu） and gama-aminobutyric acid （GABA） of the hippocampus were measured by high-performance liquid chromatography. The concentration of corticotropin releasing hormone （CRH） , adrenocorticotropic hormone （ACTH） and cortisol （CORT） in the plasma of the rats were detected by radio-immunity. The CRHmRNA expressions in the hypothalamus and prefrontal cortex were semiquantified by RT-PCR method. The differences of BDNF and TrkB protein expression in CAI and CA3 pyramidal and dentate gyrus granule cells layers of hippocampus were investigated with immunohistochemistry technique. Results In comparision with NC group, the levels of 5-HT, and GABA of the hippocampus in MC rats reduced significantly （ P 〈 0.01 ） , and the concentration of CRH, ACTH, and CORT of the palsma and the level of Glu of the hippocampus and CRH mRNA expression in the brain increased significantly. Fluoxetine or Baisong could significantly regulate the abnormal changes of all the above. Conclusion Chronic mild unpredicted stress can affect neuroendoerine and cause depression, and Baisong tablet has antagonism against it.