白松片对慢性应激抑郁模型大鼠海马突触蛋白SYT和SYN的影响

Effect of BST on synapse protein SYT and SYN in the hippocampus of chronic stress depression in rats

目的:观察白松片(Baisongtablet,BST)对慢性应激模型大鼠海马神经元囊泡纤夫蛋白(synaptotagmin,SYT)、突触融素(synaptophysin,SYN)的影响。方法:成年Sprague-Dawley雄性大鼠28只按体质量分层随机分为空白对照组、慢性应激抑郁模型对照组、氟西汀对照组及白松片试验组,每组7只。除空白对照组外,其余各组均给予慢性轻度不可预见性刺激。采用原位杂交及免疫印迹方法观察各组大鼠脑内SYT和SYN在海马区域的分布以及表达量的差异。结果:原位杂交及免疫印迹结果显示模型组大鼠海马SYTmRNA,SYNmRNA及蛋白表达量明显下降,与空白对照组比较差异有统计学意义(P<0.05);氟西汀组、白松片组大鼠海马SYTmRNA,SYNmRNA及蛋白表达量显著增加,与模型组比较差异有统计学意义(P<0.05)。结论:抑郁模型大鼠海马神经元内的突触可塑性下降,白松片能上调SYT,SYN蛋白及其mRNA的表达水平。

Objective To explore synatotagmin （SYT） and synaptophysin （ the effects of Baisong tablets （BST） on synapse protein SYN ） of hippocampus in chronic stress depression in rats. Methods Twenty eight male Sprague-Dawley rats were randomly allocated to 4 groups： a normal control group, a model group, a fluoxetine （FXT） group and a BST group. The normal control rats were fed in a natural environment. Rats of the model, FXT and BST groups were singly housed and given an chronic unpredicted sequence of mild stressors. The distribution and expression differences of SYT and SYN in the hippocampus of rats in different groups were investigated with in situ hybridization and immunoblotting. Results Expressions of SYT and SYN in the hippocampus of model rats were significantly reduced, compared with that of the normal control （ P 〈 0.05 ） ; and the expressions of SYT and SYN were significantly increased in the hippocampus of the FXT and BST groups, compared with that of the model group （ P 〈 0.05 ）. Conclusion The expressions of SYT and SYN protein and their mRNA decrease in the hippocampus of stress-model rats. BST can up-regulate their expression.