MMP-3,OPN和TIMP-1在白内障形成中的作用

Effects of matrix metalloproteinase-3, osteopontin,and tissue inhibitor of metalloproteinase-1 in the formation of cataract

目的:研究基质金属蛋白酶-3(matrixmetalloproteinase-3,MMP-3)、骨桥蛋白(osteopontin,OPN)和金属蛋白酶组织抑制因子-1(tissueinhibitorofmetalloproteinase-1,TIMP-1)在白内障晶状体上皮细胞中的表达及意义。方法:采用免疫组织化学方法测定MMP-3,OPN和TIMP-1在31例前囊下性白内障,28例核性白内障和12例正常晶状体上皮细胞中的表达。结果:MMP-3在前囊下性白内障的表达高于核性白内障、正常晶状体(χ2=31.49,34.479;均P=0.000),而在核性白内障与正常晶状体中无明显差别(χ2=2.449,P=0.118);OPN在前囊下性白内障的表达高于核性白内障和正常晶状体(χ2=29.450,15.889;均P=0.000);TIMP-1表达在3组晶状体上皮细胞中差异均无统计学意义(P>0.05);前囊下性白内障MMP-3和OPN的表达水平相关(r=0.381,P=0.035),而MMP-3和OPN与TIMP-1的表达水平不相关(r=0.121,-0.289;P=0.516,0.114)。结论:MMP-3和OPN的表达增强及MMP-3/TIMP-1的表达失衡可能与前囊下性白内障的形成密切相关。

Objective To investigate the expression and significance of matrix metalloproteinase-3 （ MMP-3 ）, osteopontin （ OPN ） and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） in human lens epithelial cells （LECs） of cataract. Methods The MMP-3, OPN, and TIMP-1 expressions in LECs of anterior subcapsular cataract （31 cases ）, nuclear cataract （28 cases ） and normal lens （12cases） were detected by immunohistochemistry, respectively. Results The MMP-3 expression in anterior subcapsular cataract was significantly higher than that in nuclear cataract and normal lens （ Χ^2 = 31.49, 34. 479 ; P = 0. 000 ） ; but there was no statistic significance between nuclear cataract and normal lens （ Χ^2 = 2. 449, P = 0. 118 ）. The OPN expression in anterior subcapsular cataract was also significantly higher than that in nuclear cataract and normal lens （ Χ^2 = 29.450, 15. 889; P =0. 000）. There was no significant difference in the TIMP-1 expression among the 3 groups （ P 〉 0.05 ）. Positive correlation was found between MMP-3 and OPN in LECs of anterior subcapsular cataract （ r = 0.381 , P = 0.035 ）. But no significant correlation was found among MMP-3, OPN, and TIMP- 1 （ r = 0. 121 , - 0. 289 ; P = 0.516,0. 114 ）. Conclusion Increased expression of MMP-3 and OPN and the expression imbalance between MMP-3 and TIMP-1 may play a critical role in the pathogenesis of anterior subcapsular cataract.