VEGF基因转染骨髓间充质干细胞对大鼠心梗区血管新生的影响

Angiogenic effect of bone marrow mesenchymal stem cells transfected with human VEGF gene on myocardial infarcts in rats

目的:探讨转染血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)基因的骨髓间质干细胞(mesenchymalstemcell,MSC)心肌移植及其对大鼠心梗区血管新生的影响。方法:以Wistar近交系大鼠建立心肌缺血模型,实验动物随机分为4组(n=12)。冠脉结扎2周后,联合组(A组)于心梗区移植转染VEGF基因的MSC,细胞组(B组)移植等量的MSC,基因组(C组)注射脂质体-pcDNA3.1-VEGF165DNA复合物,对照组(D组)注射等容积培养液,移植4周后通过Ⅷ因子染色检测血管新生,RT-PCR检测VEGF基因的体内表达。结果:Ⅷ因子染色示A组动物心梗区毛细血管密度明显高于B组和D组,较C组亦有一定程度的升高;RT-PCR显示VEGF基因的体内表达从高到低依次为A组、C组、B组、D组。结论:BMMSC有利于VEGF基因的稳定表达,可作为VEGF基因的良好细胞载体。

Objective To evaluate the angiogenic effect of the bone marrow mesenchymal stem cells （ MSCs ） transfected with human vascular endothelial growth factor （ VEGF165 ） gene on myocardial infarcts in rats. Methods The animal model of heart ischemic was established by ligating the left anterior descending coronary artery in Wistar rats. The ligated rats were divided into 4 groups （n = 12 each）, and 2 weeks later they were injected hVEGF-transfected MSC at the heart infarct zone （ Group A ）, MSC （ Group B ）, liposome-hVEGF gene plasmid （ Group C ）, and medium （ Group D ） . Four weeks after the injection, the capillary density of the infracted zone and the expression of human VEGF in vivo were examined. Results Four weeks after the transplantation, the capillary density was significantly greater in Group A than that in Group B and Group D, slightly greater than that in Group C. The highest expression of hVEGF was Group A, and followed by Group C, Group B, and Group D. Conclusion MSC is helpful for the stable expression of hVEGF gene, and is an ideal cellular vehicle for VEGF genes.