三种稻飞虱成虫体内酵母类共生菌的形态差异

MORPHOLOGICAL DIFFERENCE OF THE YEAST-LIKE ENDOSYMBIOTES IN ADULT PLANTHOOPERS OF NILAPARVATA LUGENS (STL), LAODELPHAX STRIATELLUS (FALL?N) AND SOGATELLA FURCIFERA (HORVATH)

试验采用冷冻切片技术结合显微摄像系统观察法研究了酵母类共生菌在3种稻飞虱成虫体内的存在状态、形态特性以及种类和组成。光学显微镜观察证实,3种稻飞虱成虫的头部和胸部均未观察到共生菌,在其腹部脂肪体中有大量酵母类共生菌,且以出芽进行无性繁殖,并伴有菌胞的出现。3种稻飞虱成虫体内共生菌的形态和组成明显不同。其中,褐飞虱体内的共生菌个体较大,以梭形、杆状和卵形为主,分别占共生菌总数的30·7%、53·5%和15·1%;灰飞虱和白背飞虱体内的共生菌个体较小,以卵形为主,分别占共生菌总数的93·4%和94·7%。此外,褐飞虱成虫体内的各类共生菌都显著大于灰飞虱和白背飞虱成虫体内的同种类型个体,且灰飞虱和白背飞虱体内的同种共生菌个体大小之间也存在一定差异。

Distribution, morphological characteristics, species constitute of yeast-like endosymbiotes （ YLES ） in the adult planthoppers of brown planthopper （ BPH ） Nilaparvata lugens （ Stal ）, smaller brown planthopper （SBPH） Laodelphax striatellus （Fallén）, and white backed planthopper （ WBPH ） Sogatella furcifera （ Horvath ）（Homoptera, Delphacidae） were studied by the cryostat microtome method in combination with the photomicrography. Light micrographs confirmed that there were no YLES in neither of cephalosome and baenosome of the adults of BPH, SBPH or WBPH. However, mass YLES and some mycetocytes were found existing in abdomen fat body of the adults of these three species of rice planthoppers, and YLES reproduces by budding proliferation. There was obvious difference in morphological characteristics and species constitute of YLES in adult planthoppers among N. lugens, L. striatellus and S. furcifera. It was mainly fusiform （30.7%）, bacilliform （53.5%） and oval （15.1%） forms to constitute the YLES in BPH,and oval forms in SBPH （93.4%） and WBPH （94.7%）. Moreover, the YLES in adults of BPH are all larger than those respective fbrms of YLES in adults of SBPH and WBPH, and there was some difference in YLES size between SBPH and WBPH for same type of YLES.