耐辐射奇球菌Deinococcus radiodurans中非编码RNA

NON-CODING RNA IN Deinococcus radiodurans

耐辐射奇球菌(Deinococcus Radiodurans)对电离辐射、紫外线以及强氧化剂等方面具有惊人的抗性。非编码RNA(non-coding RNA,ncRNA)在参与转录调控、RNA的加工与修饰、mRNA的转录、蛋白质的运输与稳定性调节等方面具有非常明显的作用。本文通过概率上下文无关算法(Stochastic ContextFree Grammar,SCFG),对耐辐射奇球菌R1菌株的基因间序列进行相似二级结构预测,结果发现,在耐辐射奇球菌R1基因组的非编码区存在28个非编码RNA家族。其中IRE家族成员最多,其次是Histone3、tRNA和SECIS家族。所发现的DicF与ctRNA_pGA1t、mRNA等家族成员为细菌所特有。与其他生物比较分析结果显示,可以用这些非编码RNA来研究耐辐射奇球菌的生物学功能,并为进一步研究其DNA损伤与修复分子机制提供依据。

Researches on DNA damage and repair pathways of Deinococcus radiodurans show its extreme resistance to ionizing radiation, ultraviolet radiation and reactive oxygen species. Non-coding （ncRNA） RNAs are involved in a variety of processes such as transcriptional regulations, RNA processing and modification, mRNA translation, protein transportation and stability. The conserved secondary structures of intergenic regions of Deinococcus radiodurans R1 were predicted using Stochastic Context Free Grammar （SCFG） scan strategy. Results showed that 28 ncRNA families were present in the non-coding regions of the genome of Deinococcus radiodurans R1. Among these families, IRE is the largest family, followed by Histone3, tRNA, SECIS. DicF, ctRNA_ pGA1 and tmRNA are only discovered in bacteria. Results from the comparison with other organisms showed that these ncRNA can be applied to the study of biological function of Deinococcus radiodurans and supply reference for the further study of DNA damage and repair mechanisms of this bacterium