高效液相色谱-蒸发光散射检测法测定花芪通脉颗粒中黄芪甲苷的含量

Determination of Astragaloside IV in Huaqitongmai Granule by HPLC-ELSD

目的建立测定花芪通脉颗粒中黄芪甲苷含量的方法。方法采用高效液相色谱-蒸发光散射检测（HPLC-ELSD）法，固定相：ZORBAX-C18（4．6mm×150mm，5μm）色谱柱；流动相：乙腈-水（35：65）；流速：0．8mL-min^-1；柱温：30℃；蒸发光散射检测器参数：漂移管温度为105℃，载气为氮气，气体流速为2．70mL·min^-1，压力为2BAR。结果黄芪甲苷在1．53-7．64μg范围内与峰面积呈良好的线性关系（r=0．9996），黄芪甲苷平均加样回收率为98．6％，RSD：1．17％（n：5）。结论该检测方法可靠、准确、重现性好，适用花芪通脉颗粒中黄芪甲苷的含量测定。

Objective To describe a simple and rapid HPLC-ELSD method for the baseline separation, identification and assay of astragaloside IV in huaqitongmai granule . Methods The analytical column was a ZODBAX-C18 （4.6 mm × 150 mm,5 μm）. The mobile phase was composed of acetonitril-water（35 ： 65）. The flow-rate was 0.8 mL · min^-1. The column temperature was 30 ℃. The injection volume was 20 μL. The temperature of drift tube for ELSD was 105℃ and the flow rate of N2 was 2.70 mL · min^-1. The pressure was 2 BAR. Results The calibration curve was linear over the range of 1.53 - 7.64 μg （ r = 0.999 6）. The average recovery of the method was 98.6%, and RSD was 1. 17% （ n = 5 ） . Conclusion The proposed method would be used in the quantitative analysis of astragaloside IV in huaqitongmai granule and the method is convenient, economical and accurate with good reproducibility.