玉米蛋白粉的剪切乳化处理及酸性蛋白酶的水解研究

探讨了高速剪切乳化预处理对提高玉米蛋白粉酸性蛋白酶酶解效果的作用。结果表明,利用高速剪切乳化方法在7000r/min下预处理玉米蛋白粉达1h,可使玉米蛋白粉的颗粒度从420μm降到10μm左右。在此情况下,采用酶活力为50000u/g的酸性蛋白酶在50℃,酶与底物比10%,底物浓度80g/L和水解时间24h的水解条件下(不加碱维持水解液的pH),可将玉米蛋白粉的降解率和蛋白质水解度分别提高到70.19%和58.64%。

In this paper, the effect of high -speed emulsification pretreatment on the acidic protease catalyzed hydrolysis of corn gluten meal（CGM）is investigated. Results show that under the action of emulsification equipment at 7000 r/min for one hour, the average particle size of CGM can be decreased from 420μm to approximately 10μm. When the pretreated CGM is subjected to acidic protease catalyzed hydrolysis under conditions of 50℃, acidic protease/substrate ratio 10%（g/g）, CGM substrate content 80g/L and hydrolysis time 24 h, the degradation percentage can reach 70.19% ,while the hydrolysis degree can reach 58.64%.