β-葡聚糖酶分级降解魔芋葡甘聚糖工艺研究

对β-葡聚糖酶分级降解魔芋葡甘聚糖工艺进行了研究。结果表明,β-葡聚糖酶降解KGM的效果优于纤维素酶;用β-葡聚糖酶分级降解,可以基本控制降解产物的分子量范围;用不同浓度乙醇溶液对降解产物进行分级沉淀,对不同分子量的降解产物也有一定的分离纯化作用。

Limited hydrolysis of konjac glucomannan （KGM） with β-glucanase was studied. The results showed that β-glucanase was better than oellulase for the hydrolysis of KGM. The molecular weight range of the hydrolysate could basically be controlled by limited hydrolysis, And the hyclrolysate of different molecular weights could be purified to a certain extent by grading precipitation with varying ethanol concentration.