粗江蓠多糖的提取及光谱分析

Extraction and Analysis of Gracilaria Gigas Harvey Polysaccharides

采用热水浸提和乙醇沉淀的方法提取粗江蓠多糖(gracilaria gigas harvey polysaccharides,GHPS),用苯酚-硫酸法测定糖含量,等离子体原子发射光谱(ICP)测多糖中矿物元素的含量,傅里叶红外光谱仪(FTIR)分析糖类官能团。结果表明:采用热水浸提和乙醇沉淀的方法提取粗江蓠多糖提取率达14.98%,苯酚-硫酸法测得提取物多糖含量为78.2%;ICP测得粗江蓠多糖含Ca,Fe,Mg和S等矿物元素;FTIR分析GHPS有一般糖类物质的特征吸收峰,是一种酸性多糖,同时存在呋喃环和吡喃环。

Gracilaria Gigas Harvey Polysaccharides （GHPS） were extracted with hot water and sedimentated with ethanol. The amount of polysaccharides was determined by phenol-H2SO4 method. The mineral elements and molecular configuration were analysed by inductively coupled plasma （ICP） and infrared absorption spectrum （IR）. The rate of distillation was 14.98%, the amount of polysaccharides was 78.2%, and GHPS contained manifold mineral elements （Ca, Fe, Mg and S）. The IR spectra manifested that the extraction was a compound of polysaccharides, i.e. acidic polysaccharides.