双温聚合酶链反应检测喉乳头瘤中HPV－DNA

^Detection of HPV DNA in Laryngeal Papilloma Using two Temperature PCR

应用双温聚合酶链反应（ＰＣＲ）检测喉乳头瘤石蜡包埋组织中的人乳头瘤病毒（ＨＰＶ）ＤＮＡ。结果与常规ＰＣＲ（三温循环）相同：３０例喉乳头瘤中ＨＰＶ总检出率为４６．７％（１４／３０），ＨＰＶ－６／１１／１６的检出率分别为４０％（１２／３０）、１６．７％（５／３０）和６．７％（２／３０），ＨＰＶ－６的检出率极显著高于ＨＰＶ－１１／６（ｘ２＝１０．６，Ｐ＜０．０１）。初步结果表明，本地区喉乳头瘤主要与ＨＰＶ－６的感染密切相关。该法具有缩短检测时间、节省一个恒温水浴箱等优点。

human papillomavirus (HPV)DNA in 30 paraffinembedded specimens from laryngeal papilloma (LP) was detected by using two temperature polymerase chain reaction (PCR).The same results could be obtained as by routine PCR method.The total positive rate of HPVs DNA was 46.7%(14/30).The positive rates of HPV-6,HPV-11 and HPV-16 were 40% (12/30), 16.7%(5/30),6.7%(2/30), respectively.The positive rate of HPV -6 was markedly higher than that of HPV-11 and HPV-16 (p<0. 05 and p<0. 001). HPV-18 DNA was not found. The results suggested that HPV-6was predominantly related to LP in the Shantou area.This study also indicated that detection of HPV DNA by two temperature PCR was much faster and simpler than that by routine PCR method,The two temperature PCR method is worth popularizing in the primary laboratory and clinic for the detection of HPV DNA.