荧光定量聚合酶链反应检测人乳头瘤病毒的实验研究

EXPERIMENTAL STUDY ON DETECTION OF HUMAN PAPILLOMA VIRUSE WITH FLUORESCENT QUANTIVATIVE PCR(FQ-PCR).

目的:探讨尖锐湿疣(CA)患者人乳头瘤病毒(HPV)的感染情况及荧光定量聚合酶链反应(FQ-PCR)检测HPV与分型的临床意义。方法:用FQ-PCR测定292例CA患者疣体组织或分泌物的HPV6、11和HPV16、18及HPV-DNA载量。结果:292例CA患者标本中HPV-DNA阳性感染率95.55%,其中HPV6、11型感染率85.62%,HPV16、18型感染率22.95%,HPV6、11型和HPV16、18型混合感染的感染率为13.01%。定量测定结果显示:HPV6、11型患者病毒载量最高1.0×108copies/ml,最低2.8×103copies/ml;HPV16、18型患者病毒载量最高1.0×108copies/ml,最低2.75×104copies/ml。结论:FQ-PCR检测技术具有灵敏、简捷、安全、特异性等的特点,其定量检测的结果可为临床治疗及观察CA患者感染HPV后病情变化提供可靠依据,有较高的临床实用价值。

Objective： To study the infection situation situation of HPV of CA patients and evaluate the significance of FQ- PCR method in the diagnosis of CA and the typing of HPV. Methods： The fixed quantity of DNA and the type of HPV was detected for 292 CA patients by FQ- PCR. Results： In 292 examples of CA, HPV was found in 279 examples; HPV6,11 was found in 250 examples and HPV16,18 was found in 67 examples, HPV6,11 and HPV16,18 was in 38 examples. This detection of fixed quantity showed： patients of IHPV6,11 had 1.0 × 10^8 copy max and 2.8 × 10^3 copy rain, and the ones of HPV16,18 had 1.0 × l0^8 copy max, 2.75 × 10^4 copy rain. Conclusion： The technique of FQ- PCR is provided with merits： high- sensibility, efficiency, and avoid of feignd- positive conditions due to purely enhanced production of PCR. Additionally the quantitative results were useful to support strong information for the process of clinical treatment.