高效液相色谱法测定妇科消炎0号颗粒剂中大黄素和大黄酚的含量

Determination of emodin and chrysophanol in granules for relieving inflammation in women ( Fukexiaoyanlinghao granule) by HPLC

目的:建立妇科消炎0号颗粒剂中大黄素和大黄酚含量测定的方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS2柱(250 mm×4．6 mm,5μm),流动相为甲醇-0．1％磷酸(83:17),波长为254 nm。结果:大黄素的线性范围为0．04～0．45μg,r=0．999 9;大黄酚的线性范围为0．04～0．48μg,r=0．999 9;大黄素和大黄酚的平均加样回收率分别为100．21％～101．45％,95．50％～95．95％,RSD分别为0．18％～1．2％,0．18％～0．70％(n=3)。结论:用高效液相色谱法测定妇科消炎0号颗粒剂中大黄素和大黄酚含量,方法准确、简便易行。

Objective ：To establish a HPLC method for determination of emodin and chrysophanol in granules for relieving inflammation in women（Fukexiaoyanlinghao granule）. Methods： The quantification was performed by HPLC consisted of a Hypersil ODS2 column（250 mm ×4.6 mm,5μm） , an eluate of methanol-0. 1% phosphoric acid（83： 17）and a UV detector at 254 nm. Results： The linearity of emodin and chrysophanol concentrations curves has in a range of 0.04 - 0.45 /μg （ r = 0. 999 9） and 0.04 -0.48μg（r =0. 999 9） ,respectively. The mean recovery rate of the emodin and chrysophanol was 100.21% -101.45% （RSD 0.18% - 1.2%）versus 95.50% -95.95% （RSD 0.18% -0.70%）. Conclusion ： The simple and reliable HPLC method can be used for quality control of Fukexiaoyanlinghao granule.