鸡新城疫水－油－水型活性复乳的研制和免疫研究

Preparation and Immune Effect of W/O/W Active Multiple Emulsion against Newcastle Disease Virus

水－油－水（Ｗ／Ｏ／Ｗ）型活性复乳是将油包水初乳（Ｗ／Ｏ）进一步分散在水相中经过二次乳化，使用时并配以新城疫弱毒疫苗联合制成。采用透析法测定复乳的形成率分别为９５．０％，８９．３％，８８．０％和８４．４％，雏鸡免疫后４ｄＨＩ抗体即明显升高，井持续２个月以上；对育成鸡的加强免疫持续期达１４个月，滴鼻或肌注攻毒全保护，证实新城疫Ｗ／Ｏ／Ｗ活性复乳已达到实用要求。

Newcastle disease (ND) is a serious infectious disease for the poultry industry in China,and occurres frequently though immunization of active and inactive vaccine against ND has been widespread applied. In this paper, W/O/W active multiple emulsion (multiemulsion) has been prepared at first by way of second emulsifying in exophase of water with primary oil emulsion (W/O) of inactive vaccine against ND, and active vaccine is added when immunization will be carried out.The results show: the forming percentage of the multiple emulsion are respectively 95. 0, 89. 3,88. 0 and 84. 4 using Permeability's method. HI antibody titers against NDV raise obviously after immuned 4 day and maintain up to 2 months for young chickens, and the immune effect could maintain up to 14 months after reinforcing immune for three-month-old chickens. The protected rate of immuned chickens using W/O/W active multiple emulsion are all 100% for challenge of NDV.