黄曲霉毒素高污染区肝细胞癌P53基因高频率定点突变

High Frequency of P53 Gene Fixed Point Mutation in Hepatocellular Carcinoma from AFB1 High Risk Area

用聚合酶链反应及限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）分析技术，对广西黄曲霉毒素高污染区５２例肝细胞癌（ＨＣＣ）进行分析，检查Ｐ５３基因第７外显子的２４９密码子的突变频率．结果发现３６／５２例ＨＣＣ中２４９密码子有集中的点突变，频率为６９．２％。Ｐ５３基因突变热点与乙型肝炎病毒感染无关。提示黄曲霉毒素Ｂ１是突变热点的主要原因。

Fifty two cases of hepatocellular carcinoma (HCC) from high aflatoxin B1 (AFB1) contaminated area in Guangxi were studied by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) analysis to examine the exon 7 of P53 gene in HCC. Mutation points were mostly clustered at codon 249 to form a mutational hot-spot. The frequency was 36/52 (69. 2%). It seems that there has no relation to hepatitis B virus infection. The result indicates that AFB1 is the most important agent for the specific mutation at hot-spot in the P53 gene of human hepatocellular carcinoma.