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生物素标记L-选择素cDNA探针检测小型猪白细胞L-选择素mRNA表达 被引量:1

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摘要 光敏生物素标记含目的基因的质粒cDNA,用DNAase随机切成100bp大小标记探针与小型猪心肌缺血模型中白细胞,在适当条件下进行杂交,测定白细胞L—选择素mRNA的表达水平,实验结果显示:L—选择素cDNA基因探针对于小型猪缺血细胞有敏感的杂交信号,术后30分钟白细胞L—选择素mRNA由阴性转化为阳性,此后表达水平逐渐降低,实验结果为临床心肌缺血患者围手术期的内科保障提供理论参考。
出处 《当代医师》 1996年第9期15-16,共2页
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  • 1[1]Rose DK, Cohen MM, DoBoer DP. Cardiovascular events in the postanesthesia care unit. Contribution of risk factors.Anesthesiology, 1996,84: 772-781.
  • 2[7]Nurmohamed MT, Buller HR, ten Cate JW. Physiological changes due to age. Implications for the prevention and treatment of thrombosis in older patients. Drugs Aging,1994,5: 20-33.
  • 3[8]van Gorp EC, Brandjes DP, ten Cate JW. Rational antithrombotic therapy and prophylaxis in elderly, immobile patients. Drugs Aging, 1998,13: 145-157.
  • 4[11]Naesh O, Friis JT, Hindberg I, et al. Platelet function in surgical stress. Thromb Haemostas, 1985,54: 849-852.
  • 5[15]Droste C, Roskamm H. Experimental pain measurement in patients with asymptomatic myocardial ischemia. J Am Coll Cardiol, 1983,1:940-945.
  • 6[16]Wallace A, Layug B, Tateo I, et al. Prophylactic atenolol reduces postoperative myocardial ischemia. Anesthesiology,1998, 88:7-17.
  • 7[17]Warltier DC. Beta-adrenergic-blocking drugs: incredibly useful, incredibly underutilized. Anesthesiology, 1998, 88:2-5.
  • 8[18]Gaziano JM, Skerrett PJ, Buring JE. Aspirin in the treatment and prevention of cardiovascular disease. Haemostasis,2000,30(Suppl 3): 1-3.
  • 9[19]Schwartz L, Bourassa M, Lesperance J, et al. Aspirin and dipyridamole in the prevention of restenosis after percutaneous transluminal coronary angioplasty. N Engl J Med,1988,318:1714-1719.
  • 10[20]Goldman S, Copeland J, Moritz T, et al. Saphenous vein graft patency 1 year after coronary bypass surgery and effects of antiplatelet therapy: result of a Veterans Administration Cooperative Study. Circulation, 1989, 80: 1190-1197.

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