用于乙肝表面抗原及其抗体同步检测压电免疫传感器阵列的研制

Development of a piezoelectric immunosensor array for detection of HBsAg and anti-HBs

目的：研制同步检测HBsAg和anti—HBs的压电免疫传感器阵列。方法：采用戊二醛交联法，将乙肝表面抗体及其抗原固定于石英晶体表面，同步检测HBsAg和anti—HBs。结果：该阵列对HBsAg和anti—HBs能进行定性定量分析，可以明显区分阴阳性对照血清，以阳性频移值为信号，阴性频移值为噪声，其信噪比均大于2；其线性范围分别为1～25μg／ml和0．032～1．6mg／ml。结论：该阵列具有临床推广应用价值。

Objective：To develop a piezoelectric immunosensor array for the detection of HBsAg and anti - HBs. Methods：Using glutaraldehyde cross - linking method, the antibody and antigen were immobilized on the electrode surface. A piezoelectric immunosensor array for the detection of HBsAg and anti - HBs was developed. Results： The array can analyse HBsAg and anti - HBs qualitatively and quantificationally. It can distinguish feminine and masculine serum obviously. As the frequency shifts of masculine being signal and as frequency shifts of feminine being noise, the Signal - to - Noise was bigger than 2. The linearity of HB- sAg and anti - HBs in the range of 1 -25μg/ml, 0. 032 - 1.6 mg/ml was obtained respectively. Conclusion：The array is worthy for extending in clinic.