液相色谱-电喷雾串联质谱内标法测定水产品中氯霉素药物残留

Determination of chloramphenicol in aquatic products with internal standard by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

目的：建立水产品中氯霉素药物残留的LC—MS／MS分析方法。方法：以乙酸乙脂为提取溶剂，经匀质、正己烷脱脂、用Lc—MS／MS、同位素内标法测定水产品中氯霉素药物残留。结果：该法测定检出限0．1μg／kg，回收率为95％～110％。结论：该法可适用于水产品中氯霉素药物残留的确证检验。

Objective：To develop a method for determination of ehloramphenieol （CAP） in aquatic products with internal standard by liquid chromatography coupled to eleetrospray ionization tandem mass spectrometry. Methods：Sample spiked with an isotopieally labelld internal standard （d5 -CAP） was homogenized with ethyl acetate followed by defatted with hexane and analysed by LC - MS/MS. Results ： The limit of quantitation was 0. 1μg/kg, the recovery was 95% to 110%. Conclusion ： The method can be used for quantitation and confirmation of the CAP residues in aquatic products.