异种血清对猪血管内皮细胞适应模型的影响

Influence of xeno-serum on accommodation model of porcine aortic endothelial cell

目的:观察胎牛血清对PAEC适应模型的影响。方法:以猪自体血清原代培养PAEC并传代,接种于96孔板后分别以5%正常人血清(NHS组)、胎牛血清(FCS组)和补体灭活的胎牛血清(FCS+C组)诱导6d,加入25%正常人血清,以MTT比色法检测细胞的存活率,并以免疫组化的方法观察各组HO-1的表达情况。结果:NHS组和FCS+C组细胞存活率均升高,同对照组相比差异显著,两组间亦有差异,HO-1表达均增加。FCS组与对照组无差异。结论:质量不佳的胎牛血清或补体灭活不充分的胎牛血清会干扰PAEC适应模型的诱导。

Objective：To observe the influence of fetal calf serum on accommodation model of porcine aortic endothelial cell （PAEC）. Methods ： The primary PAEC had been cultured and passage with porcine autoserum. PAECs were plated in 96-well microculture plates, and incubated with 5 %AB normal human serum （NHS）, 5% heat-inactivated FCS and 5% no heat-inactivated FCS for 6 days respectively, then the survival rates were detected by MTT chromatic test and expression of heme oxygenase 1 （HO-1） was observed by immunohistochemical method after these PAECs had been incubated with 25 % NHS for 2 hours. Result： The survival rates of the PAEC incubated with 5% NHS and 5% no heat-inactivated FCS increased compared with control group, and there was difference between the two groups also. There was not statistical significance between heat-inactivated FCS group and control group. The expression of HO-1 was upregulated in NHS group and FCS＋C group. Conclusion ：Low quality fetal calf serum , or not fully heat-inactivated FCS may influence the induction of accommodation of in vitro cultured PAEC.