纳洛酮对血管性痴呆大鼠海马神经细胞形态和超微结构的影响

Effects of Naloxone on the Morphology and Structure of Hippocampal CAl Pyramidal Neurons of Rats with Vascular Dementia

目的研究纳洛酮对血管性痴呆(vascu lar dem entia,VD)大鼠海马神经细胞形态和超微结构的影响。方法采用双侧颈总动脉永久结扎方法制备VD大鼠。随机分为3组:假手术组,VD组,VD纳洛酮治疗组(每天0.8 mg/kg纳洛酮腹腔注射,连续7 d)。采用N issl染色观察海马神经细胞形态和数量,电子显微镜观察海马神经细胞超微结构。结果VD大鼠CA1区锥体细胞数(66.21±19.34)和纳洛酮组的(101.68±20.06)与假手术组的(157.11±12.27)相比,分别显著减少,差异有统计学意义(P<0.01),其中纳洛酮组大鼠的细胞数比VD组明显增多(P<0.01)。纳洛酮组大鼠海马CA1区锥体细胞超微结构较VD组明显改善。结论纳洛酮可以减少VD大鼠海马神经细胞的丢失并改善神经细胞的超微结构。

[ Objective] To investigate the effect of naloxone （ NAL） on the morphology and structural changes of pyramidal neurons in hippocampal CA1 of rats with vascular dementia （ VD ）. [ Methods ] Vascular dementia was established by permanent occlusion of the common carotid arteries. Rates were divided into three groups： sham - operation group, VD group and VD - naloxone group （ naloxone 0.8 mg/kg, ip daily for 7 days ）. Nissl staining and electron microscope were used to observe the morphology and structure of pyramidal neurons in hipocampal CA1 of rats with vascular dementia. [ Results ] CA1 pyramidal neuron counting in the VD group （ 66.21 ± 19.34 ） was significantly lower than that in the naloxone group （ 101,68 ± 20.06 ） and the sham - operation group （ 157.11 ± 12.2 ） , the difference was significant （ P 〈 0.01） ; the neuron counting in the naloxone group was obviously higher than that in the VD group （ P 〈 0.01）. CAI pyramidal neurons ultramicro structure in the naloxone group was obviously improved com- pared with the VD group. [ Conclusion] Naloxone could inhibit the loss of hippoeampal CA1 pyramidal neurons in rats with VD and improve the ultramicro structure of neurons.