甘草酸二铵对溃疡性结肠炎大鼠促炎细胞因子IL-6、IL-8、TNF-α的影响

Effects of diammonium glycyrrhizinate on expression of IL-6,IL-8 and TNF-α in rats model with ulcerative colitis

目的建立溃疡性结肠炎(UC)大鼠模型,研究甘草酸二铵(DG)对大鼠溃疡性结肠炎的治疗作用及对肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)、白细胞介素8(IL-8)产生和表达的影响。方法30只雌性SD大鼠随机分为实验组、模型组和对照组各10只,建立UC急性期模型,实验组给予DG干预治疗。观察疾病活动指数(DA I)、结肠大体形态损伤、组织学变化;EL ISA法测定血清TNFα-、IL-6、IL-8水平。结果与模型组相比,实验组的DA I、结肠大体形态损伤、组织学变化显著改善(P均<0.01),血清TNFα-、IL-6、IL-8水平显著降低(P均<0.01)。结论DG对大鼠UC有显著的治疗作用,抑制促炎因子TNFα-、IL-6、IL-8的产生和表达可能是其治疗机制之一。

[Objective] To investigate the regulatory effects of diammonium glycyrrhizinate （DG） on proinflammatory cytokines TNF-α, IL-6 and IL-8 in rats model with ulcerative colitis （UC）. [Methods] 30 Spragur-Dawley （SD） female rats were randomized into treatment group, acetic acid control group and normal control group, 10 rats in each group. Colitis rats in treatment group and acetic acid control group were induced by administrating acetic acid rectally. The rats in treatment group were treated with DG. Colon mucosal inflammation and damage were assessed by measuring disease activity index （DAI） mucosal lesion area and histological damage scores. Levels of serum interleukin-6 （IL-6）, interleukin-8 （IL-8） and tumor necrosis factor-alpha （TNF-α） were detected by ELISA. [Results]Compared with the acetic acid control group, the DAI, mucosal lesion area and histological damage scores in treatment group were significantly improved,and the serum levels of IL-6, IL-8 and TNF-α of treatment group were significantly decreased （P〈0. 01）. [Conclusion]The present study proved that DG has therapeutic effect on UC, it can down-regulate the serum levels of IL-6, IL-8 and TNF-α.