多氯联苯(PCB_(1254))对异育银鲫血清和离体卵巢分泌睾酮和雌二醇的影响

Effects of polychlorinated biphenyls(PCB_(1254)) on testosterone and estradiol of serum and culture ovary in allogynogenetic silver crucian carp

采用活体注射和卵巢体外培养添加多氯联苯(PCB1254)两种方式研究了PCB1254对异育银鲫血清中和离体卵巢分泌睾酮及雌二醇的影响。注射PCB1254后,血清中睾酮浓度随作用时间延长而降低,随PCB1254浓度的增加而减小;血清中雌二醇随作用时间延长而增加,随PCB1254浓度增加而增加。体外培养时卵巢时,培养液中雌二醇含量随培养时间延长而增加,随PCB1254浓度增加而增加。结果表明PCB1254促进雌二醇分泌,抑制睾酮分泌。

Environmental hormones and endocrine-disrupting chemicals, are outside factors, which can interfere in the normal function of natural hormone, such as production, release, transport, metabolism, link, effect or exclusion. These polluters disordering estrogen and reproduction could link with estrogen receptor and even produce estrogen effects. As a typical kind of environmental hormones, polychlorinated biphenyls（PCBs） are a class of chlorinated hydrocarbon compounds with high chemical stabilities and toxicity, and often extant in environment and bio-accumulated in food chains, leading to a ubiquitous contamination in nature and man. In laboratory studies, technical PCB mixtures have been shown to cause reproductive disorders in fish. But the interferential mechanism of PCB was not found, the aim of the present study was to provide the research of the mechanism with the effects of PCB1254 on testosterone and estmdiol of serum and culture ovary in allogynogenetic silver crucian carp. The effects of PCB1254 on sex steroids in allogynogenetic silver crucian carp were investigated by using in vitro injection of PCB1254 0.5, 5, 50 /Lg. kg^- 1 body weight. In injection groups, the levels of testosterone in the serum decreased with time and concentration of PCB1254. Meanwhile, the levels of estradiol in the serum changed in the opposite trend. In the groups of incubated ovarian fragments in vitro with PCB1254, the levels of estradiol secretion from ovary increased with time and concentration of PCB1254 treatments.