Cyr61、CTGF和Nov基因在孤立性大肝癌中的表达及其意义

THE EXPRESSION AND SIGNIFICANCE OF Cyr61、CTGF AND Nov GENES IN SOLITARY LARGE HEPATOCELLULAR CARCINOMA

目的探讨CCN家族成员的Cyr61、CTGF和Nov基因在孤立性大肝癌、结节性肝癌、小肝癌中的表达差异及其临床意义。方法比较孤立性大肝癌、结节性肝癌、小肝癌的临床病理特征,采用半定量的RT-PCR方法分析各组肝癌组织中Cyr61、CTGF和Nov基因的表达,并分析其表达临床病理特征的关系。结果83.3%(10/12)的结节性肝癌有静脉浸润,明显高于孤立性大肝癌和小肝癌(P<0.05),结节性肝癌的分化程序和肝硬化程度也明显较孤立性大肝癌和小肝癌差(P<0.05),孤立性大肝癌和小肝癌间的病理指标比较无显著性差异(P>0.05)。孤立性大肝癌中Cyr61和CTGF基因表达量均较结节性肝癌低,差异有显著性意义(P<0.01)。结论孤立性大肝癌的临床病理特征较好,且Cyr61、CTGF基因在孤立性大肝癌中的表达明显低于结节性肝癌,提示孤立性大肝癌有相对较好的肿瘤生物学行为。

Objective To study the expression and significance of CYR61 ,CTGF, Nov gene mRNA in solitary large hepatocellular carcinoma（SLHCC）. Methods Compare the clinopathological features of solitary large HCC and nodular hepatocellular carcinoma （NHCC）. The mRNA expression levels of CYR61 ,CTGF,Nov gene mRNA IN SLHCC and NHCC were examined semi-quantitatively and the relationship between these expression levels and clinical significance were evaluated. Results Compared to NHCC, SLHCC has better cell differentiation , capsule formation was more easily, microscopic venous invasion occurred less frequently, associated liver cirrhosis was milder. The expression of Cyr61 and CTGF gene in NHCC, were significally higher than that in SLHCC and small hepatocellular carcinoma（SHCC）. Conclusion The expression of Cyr61 and CTGF gene in NHCC were higher than that in SLHCC, suggest that the SLHCC has better biological behavior than NHCC.