猪尿中克伦特罗残留的ELISA检测研究

Study on ELISA for detecting clenbuterol residue in swine urine

对自主开发研制和德国r-Biopharm公司的p兴奋剂酶联免疫（EUSA）试剂盒的标准曲线线性范围、斜率、B／岛抑制浓度、板内变异系数、板间变异系数、阳性检出率、回收率和交叉反应率等进行了比较试验。结果表明：自制试剂盒的线性范围为0．3～8．1ng／ml时，50％抑制率浓度IC50的平均值为0．93ng／ml；板内变异系数小于2．2％；板间变异系数小于13．8％；样品回收率为85％～96％；实际样品检测和德国r-Biopharm公司的试剂盒阴阳性符合率100％；与克伦特罗和甲基克伦特罗交叉反应率均为100％，与沙丁胺醇和特布他林的交叉反应率分别为88％和86％。

The linear scope and slope of standard curve, B/B0 suppressive density, variation coefficient of inter-and intra-assay, masculine picking out rate, recovery-rate and across reactivity of the two β-agonist kits which developed by ourselves and produced from r-Biopharm company in Germany were compared. The results showed that the β-agonist kit which delved by ourselves had good sensitivity with the average IC50 value of 0.93 ng/ml when the linear detection was 0.3 - 8.1 ng/ml. The inter-assay variation coeffient was below 2.2%, the intra-assay variation coefficient was below 13.8%. The kit also showed fine precision and accuracy with the recoveries of clenbuteml spiked in swine urine were 85% - 96% and the agreement percent of detection with kit r-Biopharm were 100%. The kit generally had 100% cross-reactivity towards clenbuterol and methyl-clenbuterol, and had 88% and 86% cross-reactivity towards salbuteml and terbutaline, respectively.