早熟花椰菜小孢子高效胚胎发生及植株再生

Highly efficient microspore embryogenesis and plant regeneration in early maturing cauliflowers(Brassica oleracea var. botrytis)

以7个早熟花椰菜F1杂交品种和1个中熟花椰菜杂交品种为供体材料,对早熟花椰菜小孢子培养的胚胎发生及植株再生进行了研究。结果表明,供试材料绝大多数能诱导出胚,其胚状体产量与供体基因型有关,最高每花蕾胚状体产量达到112个。其中,‘厦雪40天’的平均每花蕾胚产量最高,达到45.6个,而‘早花45天’未能诱导出胚。小孢子胚状体萌发率一般在30%左右,其中高的达到80%,但有未能诱导出再生苗的。小孢子再生植株间在生育期及育性上存在较大变异。

Seven early-maturing hybrids and one medium-maturing hybrid of cauliflowers （ Brassica oleracea var. botrytis ） were applied to isolated microspore culture. The results showed that the microspore embryogenesis in early-maturing cauliflowers was induced in almost donor plants in spite of showing genotype-dependency from the lowest nothing to the highest 112 embryos per flower bud. The highest average embryo yield was ＇ Xiaxue 40＇, which produced 45.6 embryos per bud, while did ＇Zhaohua 45＇ null. The gemainated rate for embryos was normally around 30%, while two genotypes was over 80% but one got nothing. The great variant in growth stage and fertihty was observed in regenerated plants.