摘要
考察重组人生长激素(rhGH)可生物降解微球在动物体内的缓释效果,探讨蛋白稳定剂对微球体内药物动力学行为的影响。大鼠皮下注射rhGH-PLGA微球后,利用放射免疫吸附分析法测定血药浓度。结果表明,rhGH-PLGA微球具有明显的缓释作用,给药后d30,动物体内的rhGH浓度仍维持在正常生长激素水平之上。而大鼠同等剂量给予rhGH溶液24h后,其体内的生长激素浓度即恢复至正常水平。微球内水相中加入泊洛沙姆407(F127),使rhGH的相对生物利用度增加约12.5%,进一步证实了F127对rhGH具有稳定作用。
In order to investigate the pharmacokinetics of recombinant human growth hormone （rhGH）-loaded biodegradable microspheres, and to evaluate the effect of protein stabilizing agent on its in vivo release, rhGH-microspheres consisting of poly （lactic-co-glycolic acid） （PLGA） were administered to rats via subcutaneous injection, and serum rhGH concentration was determined using a radio immunoassay kit. The pharmacokinetic results showed that after injection the microspheres could sustain release for at least 1 month in vivo, as indicated by the rhGH serum concentration which still maintained higher than the normal growth hormone level in rats, By contrast, the serum concentration of growth hormone had already decreased to the normal level after administration of rhGH solution for 24h. The relative bioavailability of rhGH microspheres containing stabilizer poloxamer 407 （F127） increased 12.5 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2006年第11期745-748,共4页
Chinese Journal of Pharmaceuticals
基金
863项目(2001AA215451和2004AA215170)
中国博士后科学基金资助项目(2003033281)
