维持性血液透析患者淋巴细胞DNA氧化损伤的研究

Study of oxidative DNA damage in lymphocyte of MHD patients

目的通过彗星(comet)实验研究维持性血液透析(MHD)患者淋巴细胞DNA的氧化损伤程度及透析膜对其的影响。方法于2003年5月至2004年10月选择中国医科大学附属第一医院的20例接受MHD治疗的慢性肾功能不全(CRF)患者,随机分为血仿膜(HE)组和聚砜膜(PS)组。分别使用HE和PS透析12周后采集静脉血,分离淋巴细胞进行comet实验。测量彗星尾长,作为DNA氧化损伤程度的观察指标。同时设立正常对照组。结果MHD患者的彗星尾长显著长于正常对照组(P<0.05),HE组的彗星尾长显著长于PS组(P<0.05)。MHD患者的彗星尾长与血清肌酐(r=0.663,P<0.05)、尿素氮(r=0.612,P<0.05)量呈显著正相关。结论MHD患者体内DNA氧化损伤的增强与血清肌酐、尿素氮量呈正相关;从DNA氧化损伤的角度来评价透析膜的生物相容性,聚砜膜优于血仿膜。

Objective To investigate the oxidative DNA damage in lymphocyte of MHD patients and the effect of dialysis membrane on it through comet assay. Methods From 2003 -05 to 2004 - 10 we selected 20 CRF patients undergoing maintenance hemodialysis and divided the patients into HE and PS group randomly. And 7 volunteers were chosen as controls. The patients in each group had undergone hemodialysis for 12 weeks using HE or PS membrane respectively. Then we took venous blood sample and separated lymphocyte. The oxidative DNA damage was measured by comet assay. Results The comet tail length of control group, HE group and PS group was （0. 286 ＋ 0. 194） i^m, （0. 970 ＋ 0. 120） p^m and （0. 656 ＋0. 187） p^m respectively. Compared with control group, the comet tall length of MHD patients was significantly longer （ P 〈 0. 05 ）. The comet tail length of HE group was significantly longer than that of PS group （ P 〈 0. 05 ）. And there was a positive correlation between the comet tall length and the level of serum creatinine and blood urea nitrogen. Conclusion The oxidative DNA damage in MHD patients is enhanced, and this increscent is positively correlated with the level of serum creatinine and urea nitrogen. To evaluate the biocompatibility of dialysis membrane by the oxidative damage of DNA, PS membrane is superior to HE membrane.