培养时间和接种密度对咖啡短体线虫繁殖和形态的影响

Effect of Culture Time and Inoculum Density on Reproduction and Morphometrics of Pratylenchus coffeae in Carrot Disk Cultures

采用胡萝卜片培养短体线虫技术，测试不同培养时间和接种密度对咖啡短体线虫（Pratylenchus coffeae）繁殖和形态的影响。结果表明，在25℃培养条件下，每片胡萝卜（厚10mm，直径3cm）接种25头线虫雌虫，线虫的数量随培养时间的延长而增加。前3周线虫的繁殖系数为6．24，线虫的群体数量增长缓慢；第3．6周线虫的繁殖系数略有增高，为8．99；第6—9周其繁殖系数高达17．88，线虫群体数量快速增长；之后，线虫群体数量增速下降。其繁殖系数降为1．42。在接种不同密度线虫的实验中，线虫的群体数量均显著增长，其繁殖系数在不同接种密度处理中差异不显著；随机挑取10头雄虫与雌虫，比较不同线虫密度对线虫形态特征的影响，结果表明，线虫密度除对雌虫的b值（体长／头顶至食道腺与肠连接处长）有显著影响外，对雄虫和雌虫的其他形态特征测量值均无显著影响。

Effect of culture time and inoculum density on reproduction and morphometrics of Pratylenchus coffeae was evaluated with carrot disk culture method. Each carrot disk was inoculated 25 P. coffeae females, the nematode population increased with increasing culture time. The reproductive rate of nematodes is 6.24 in the first three-weeks and 8.99 in the second three-weeks. Afterwards the nematodes reproductive rate increased sharply and reached to 17.88 from the 6th week to 9th week; after the 9th week reproductive rate of nematode decreased to 1.42. The nematode population increased significantly in all the treatments of the density comparing experiment, but its reproductive rates were not significantly influenced by the nematode densities. Ten female and male nematodes were randomly picked from different density treatments, and their morphometrics were measured. All the morphometrics of female and male did not show significant differences among different density treatments except the b value （length of body/distance from anterior end to pharyngointestinal valve） of the female.