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人双突变型低氧诱导因子1α真核表达载体的构建及鉴定 被引量：1

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摘要 目的构建能在哺乳动物细胞得到常氧下高表达的携带HIF-1α突变型HIF-1α-Ala402-Ala564基因真核表达载体pShuttle2-HIF1α-Ala402-Ala564。方法采用分子克隆技术,在业已完成的pShuttle2-HIF1α-Ala564的基础上,用连续聚合酶链式反应(PCR)定点突变的方法将其第402位脯氨酸密码子CCA突变为丙氨酸密码子GCA,构建成双突变HIF-1α真核表达载体pShuttle2-HIF-1α-Ala402-Ala564。结果经酶切鉴定及基因测序证实人双突变型低氧诱导因子1α真核表达载体pShuttle2-HIF1α-Ala402-Ala564构建成功。结论成功构建重组人双突变型低氧诱导因子1α真核表达载体pShuttle2-HIF1α-Ala402-Ala564,为缺血性心脏病的HIF-1α基因治疗研究奠定基础。
作者 童锴 谢宜军 王月刚 吴平生
机构地区 南方医科大学南方医院心内科
出处 《广东医学》 CAS CSCD 北大核心 2006年第11期1650-1652,共3页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:30370587)
关键词 低氧诱导因子-1Α 突变型 真核表达载体 基因治疗
分类号 R382.31 [医药卫生—医学寄生虫学]
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参考文献10

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同被引文献10

  • 1郭寿贵,吴平生,王月刚,傅锐斌.人突变型低氧诱导因子1α腺病毒载体的构建及鉴定[J].第四军医大学学报,2005,26(17):1614-1617. 被引量：4
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广东医学
2006年 第11期

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