辣蓼中芦丁的薄层及紫外光谱鉴别

The Identification of Rutin in Laliao by TLC and UV

目的：确定辣蓼中有效成分芦丁的定性鉴别和总黄酮含量的测定方法。方法：采用薄层层析(TLC)、紫外分光光度法(UV)对辣蓼分别进行理化鉴别和含量测定。结果：芦丁确系辣蓼中主要有效成分；同时建立UV检测中浓度在0．00808-0．04848mg／ml范围内与吸收度间的具有良好线性关系,其回归方程为A=11．4716C-0．0003(r=0．9928,n=6),平均回收率为97．95％(RSD=1．88％)；辣蓼样品中总黄酮的平均含量为1．939％。结论：本方法简便、快速、准确；为辣蓼开发新药及其相关质量标准的研究提供了依据。

Objetive： To qualitatively determine rutin in Laliao, and to establish a method for the content determination of total flavonolds in Laliao. Methods： Physieochemical Properties and assaying of Laliao were identified by tile methods of thin - layer chromatography （TLC） and ultra- violet spcctrophotometry （UV）. Results： Rutin was mainly active component in Laliao. Good linear relationship with the absorbability was achieved when file detection concentration of rutin was within a range of 0.00808 -0. 04848mg/nil （r = 0.9928）, the regression equation was A = 11.4716C - 0.0003（r = 0.9928,n = 6） and the average recovery rate was 97.95% （RSD = 1.88% ）. Meanwlfile, the average content of total flavonoids was 1.939% in Laliao sample. Conclusion： Tiffs method was simple, rapid and accurate. It provided data for the exploitation and quality criteria of Laliao research.