肝硬化门脉高压症大鼠模型制作方法的探讨

Investigation of modeling portal hypertension rats with cirrhosis

目的：建立稳定可靠的肝硬化门脉高压大鼠动物模型。材料与方法：50只雄性SD大鼠,随机分为3组。正常对照组10只,行假手术,给予正常饮食。肝硬化A组20只,先行左肾上腺静脉结扎,然后给予初始浓度为0．03％的硫代乙酰胺(thioac- etamide,TAA)溶液作为饮用水并根据大鼠体重变化调节给药浓度。肝硬化B组20只,行假手术,给予固定浓度为0．03％TAA溶液。给药时间为14周。结果：肝硬化A组大鼠死亡率为0,肝硬化形成率达到100％。肝硬化B组大鼠死亡率15％,肝硬化形成率75％。肝硬化A组大鼠的门静脉压力明显高于肝硬化B组和对照组。结论：采用左肾上腺静脉结扎并根据体重变化调节TAA给药浓度可建立稳定可靠的肝硬化门脉高压大鼠动物模型。

Objective： To establish a reliable portal hypertension rat model with cirrhosis. Materials and methods： Fifty male SDrats were randomly divided into three groups： group A, B and control group. The left adrenal vein of group A（n = 20） was ligated before receiving continuous administration of thioacetamide（TAA） in the drinking water. And the concentration of TAA was adjusted according to weekly weight changes of the rats, with the initial concentration 0.03%. Group B（n= 20） was given sham operation with the fixed concentration of TAA 0. 03%. The control group （n= 10） was accepted sham operation and was supphed with normal water. Results： The mortality in group B was 15% and the rate of cirhosis formed was only 75%. In contrast, there was no death and cirrhosis formed was 100% of the rats in group A. The portal venous pressure of the rats in group A was higher than that of group B and the control group. Conclusions： The method of combining ligation of the left adrenal vein with adjustment of concentration of TAA in the drinking water according to weight changes of the rats can establish a reliable rat model of portal hypertension with cirrhosis.