摘要
目的：探讨白藜芦醇(resveratrol,Res)在体外对胃癌SGC-7901细胞VEGF表达的影响。方法：体外培养胃癌SGC- 7901细胞,MTT法检测白藜芦醇对SGC-7901细胞的增殖抑制作用,RT-PCR方法检测VEGF mRNA表达,免疫细胞化学检测VEGF蛋白的表达。结果：白藜芦醇呈时间剂量性抑制胃癌细胞SGC7901的增殖；胃癌SGC-7901细胞高水平表达VEGF,白藜芦醇能显著降低胃癌SGC-7901细胞VEGF mRNA和蛋白表达。结论：白藜芦醇可以下调胃癌SGC-7901细胞VEGF的表达,抑制胃癌细胞的增殖。
Objective： To investigate whether resveratrol could inhibit the vascular endothelial growth factor （VEGF） expression of human gastric cancer SGC - 7901 cells in vitro. Methods： Human gastric cancer SGC - 7901 cell line was cultured in vitro, proliferation inhibitory effect was tested by MTr assay; VEGF mRNA was measured quantitatively by RT - PCR methold, Expression of VEGF protein in gastric cancer cells were measured by immunohistochemistry. Results： Resveratrol could inhibit the proliferation of SGC - 79011 cells, with dose and time - related ef- fects; VEGF mRNA expressed in high level in SGC - 7901 cells, in which, resveratrol significantly decreased the expression of VEGF mRNA and protein. Conclusion： Resveratrol could inhibit proliferation and lower the expression of VEGF in human gastric cancer SGC - 7901 cell line in vitro.
出处
《现代生物医学进展》
CAS
2006年第10期52-54,共3页
Progress in Modern Biomedicine
关键词
胃肿瘤
血管内皮生长因子
白藜芦醇
Gastric cancer
Vascular endothelial growth factor（VEGF）
Resveratrol
