摘要
我所对纤溶酶原激活剂(PLGA)的测定建立了一项新方法。纤溶酶原在其激活剂作用下生成纤溶酶,该酶能特异地水解包被^(125)I-纤维蛋白底物生成可溶性的^(125)I-多肽产物。通过测定产物的放射性强度,计算出样品中纤溶酶原激活剂活性。该方法具有高度特异性和灵敏度,并且简单快速。可为凝、溶血基础研究和临床血栓病的防治服务。
A method determining the activity of plasminogen activator (PLGA) was developed in our laboratory. This method utilized the specific ability of the activated product plasmin to hydrolyze 125I labelled fibrin substrate coated on plastic cultural disc into soluble 125I-labelled polypeplide. By calculating radioactivity of the polypeptide with a γ-counter we would be able to deduce the original level of the plasminogen activator in the given sample. This method was highly specific, vevy sensistive,simple and could be easily done. It would be very useful in both research and clinical applications.
出处
《放射免疫学杂志》
CAS
1996年第6期321-323,共3页
Journal of Radioimmanology
