期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS 被引量：4

A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS
原文传递
导出
摘要 GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step of DNA replication. Although the yeast and Xenopus GINS genes are well documented, their orthologous genes in higher eukaryotes are not fully characterized. In this study, we report the genomic structure and transcriptional regulation of mammalian GINS genes. Serum stimulation increased the GINS mRNA levels in human cells. Reporter gene assay using putative GINS promoter sequences revealed that the expression of mammalian GINS is regulated by 17β-Estradiolstimulated estrogen receptor a, and human PSF3 acts as a gene responsive to transcription factor E2F1. The goal of this study is to present the current data so as to encourage further work in the field of GINS gene regulation and functions in mammalian cells. GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step of DNA replication. Although the yeast and Xenopus GINS genes are well documented, their orthologous genes in higher eukaryotes are not fully characterized. In this study, we report the genomic structure and transcriptional regulation of mammalian GINS genes. Serum stimulation increased the GINS mRNA levels in human cells. Reporter gene assay using putative GINS promoter sequences revealed that the expression of mammalian GINS is regulated by 17β-Estradiolstimulated estrogen receptor a, and human PSF3 acts as a gene responsive to transcription factor E2F1. The goal of this study is to present the current data so as to encourage further work in the field of GINS gene regulation and functions in mammalian cells.
作者 Reiko Hayashi Takako Arauchi Moe Tategu Yuya Goto Kenichi Yoshida
机构地区 Department of Life Sciences
出处 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2006年第3期156-164,共9页 基因组蛋白质组与生物信息学报（英文版）
关键词 DNA replication initiation bioinformatics gene structure gene regulation 17Β-ESTRADIOL transcription factor E2F1 DNA replication initiation, bioinformatics, gene structure, gene regulation, 17β-Estradiol, transcription factor E2F1
分类号 Q75 [生物学—分子生物学]
  • 相关文献

参考文献18

  • 1[1]Blow,J.J.and Dutta,A.2005.Preventing rereplication of chromosomal DNA.Nat.Rev.Mol.Cell Biol.6:476-486.
  • 2[2]Takayama,Y.,et al.2003.GINS,a novel multiprotein complex required for chromosomal DNA replication in budding yeast.Genes Dev.17:1153-1165.
  • 3[3]Gambus,A.,et al.2006.GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks.Nat.Cell Biol.8:358-366.
  • 4[4]Pacek,M.,et al.2006.Localization of MCM2-7,Cdc45,and GINS to the site of DNA unwinding during eukaryotic DNA replication.Mol.Cell 21:581-587.
  • 5[5]Kanemaki,M.and Labib,K.2006.Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks.EMBO J.25:1753-1763.
  • 6[6]Seki,T.,et al.2006.GINS is a DNA polymerase epsilon accessory factor during chromosomal DNA replication inbudding yeast.J.Biol.Chem.281:21422-21432.
  • 7[7]Yoshida,K.and Inoue,I.2004.Regulation of Geminin and Cdt1 expression by E2F transcription factors.Oncogene 23:3802-3812.
  • 8[8]Yoshida,K.and Inoue,I.2004.Expression of MCM10 and TopBP1 is regulated by cell proliferation and UV irradiation via the E2F transcription factor.Oncogene 23:6250-6260.
  • 9[9]Ueno,M.,et al.2005.PSF1 is essential for early embryogenesis in mice.Mol.Cell.Biol.25:10528-10532.
  • 10[10]Obama,K.,et al.2005.Up-regulation of PSF2,a member of the GINS multiprotein complex,in intrahepatic cholangiocarcinoma.Oncol.Rep.14:701-706.

同被引文献34

  • 1DE THE H,LAVAU C,MARCHIO A,et al.The PML-RAR alpha fusion mRNA generated by the t(15;17)translocation in acute promyelocytic leukemia encodes a functionally altered RAR[J].Cell,1991,66(4):675-684.
  • 2LANE A A,LEY T J.Neutrophil elastase is important for PML-retinoic acid receptor α activities in early myeloid cells[J].Mol Cell Biol,2005,25(1):23-33.
  • 3HAYAKAWA F,PRIVALSKY M L.Phosphorylation of PML by m-itogen-activated protein kinases plays a key role in arsenic trioxide-mediated apoptosis[J].Cancer Cell,2004,5(4):389-401.
  • 4SHIMA Y,SHIMA T,CHIBA T,et al.PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation[J].Mol Cell Biol,2008,28(23):7126-7138.
  • 5LANE A A,LEY T J.Neutrophil elastase cleaves PML-RARα and Is Important for the development of acute promyelocytic leukemia in mice[J].Cell,2003,115(3):305-318.
  • 6WALTHER A,HOULSTONR,TOMLINSON I.Association between chromosomal instability and prognosis in colorectal cancer:a meta-analysis[J].Gut,2008,57(7):941-950.
  • 7MACNEILL S A.Structure and function of the GINS complex,a key component of the eukaryotic replisome[J].Biochem J,2010,425(3):489-500.
  • 8TAKAYAMA Y,KAMIMURA Y,OKAWA M,et al.GINS,a novel multiprotein complex required for chromosomal DNA replication in budding yeast[J].Genes Dev,2003,17(9):1153-1165.
  • 9BAUERSCHMIDT C,POLLOK S,KREMMER E,et al.Interactions of human Cdc45 with the Mcm2-7 complex,the GINS complex,and DNA polymerases delta and epsilon during S phase.Genes[J].Cells,2007,12(6):745-758.
  • 10GAMBUS A,JONES RC,SANCHEZ-DIAZ A,et al.GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks[J].Nat Cell Bio,2006,8(4):358-366.

引证文献4

二级引证文献8

Genomics, Proteomics & Bioinformatics
2006年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部