利用大孔树脂提取辣根中硫代葡萄糖甙条件的研究

Studies on separation conditions of glucosinolates from the extraction of horseradish with macroporous resins

通过对12种大孔树脂对硫代葡萄糖甙的吸附和脱附能力的比较研究,发现大孔强碱性阴离子交换树脂D201GF选择性吸附硫甙的能力最强,且硫甙容易被洗脱,可用于从水溶液中提取分离硫甙。当利用D201GF提取硫甙时,探讨了提取液体积、pH、提取温度、吸附时间等因素对D201GF吸附硫甙的影响以及洗脱剂的种类、最小使用量、洗脱时间等因素对硫甙脱附的影响。研究结果表明,D201GF树脂的最大饱和吸附量为23.94μmol/g、吸附率96.29%;pH值对D201GF吸附硫甙几乎没有影响,不需要在吸附前对提取液进行pH值的调整,最佳提取温度50℃、振摇时间为15 min;最佳的洗脱剂是1 mol/L的NaCl,振摇30 min即可达到99%的脱附效果。

By comparison studies on 12 macroporous resins adsorbing or desorbed the glucosinolates in horseradish, D201GF that is the most strongly basic anion exchanger-resin among 12 resins, shows the higher adsorption capacity and easier desorption to glucosinolates than others. Then and it can be used to extract the glucosinolates from aqueous solution. Effects of the volume of the extraction solution, pH value, temperature and time of adsorption on the recovery of glucosinolates were studied. Meanwhile, the influences of varieties of elution, the minimum amount and time of desorption on the desorption method were also evaluated. The results show that maximum adsorption of D201GF is 23. 94μmol/g and the adsorption yield is 96. 26%. The effect of pH value was no significant. Therefore, pH-adjust is not necessary. The optimal conditions were that temperature and time of adsorption were 50℃and 15 min, respectively. The optimal eluting solvent was 1 mol/L of sodium chloride. The desorption yield is excess 99 % in the addition of temperature of 50℃ and shaking time of 15 rain.