苦瓜愈伤组织再生植株研究

Study on plant regeneration via callus culture of bittergourd(Momordica charantia L.)

将3种苦瓜的上胚轴、下胚轴、子叶、真叶和茎尖等外植体接种于脱分化诱导培养基上,均可诱导形成愈伤组织。将结构致密,乳白色或淡绿色,表面呈球状或瘤状突起的愈伤组织转移到再分化培养基上,45 d后,部分愈伤组织陆续分化形成胚状体。将胚状体转移到生根培养基上,可形成根系完整的再生植株。

The epieotyls,hypocotyls,eotyledons,euphyllus and sapoxos as explants, cut off from the sterile seedlings of Lanshan Dabai bittergourd, Qinglu Youshon bittergourd and Dading Cuilu No. 1 bittergourd, were inoculated on different dedifferentiation media and incubated at temperate of 25 - 26℃ under weak artificial light for 11 hours each day. Usually, all types of the explants anther would produce calli after 4 days. When growing up to 0.5 - 1 cm in diameter,those calli with tiny strumaes on their surface were transferred to the redifferentiation media and kept at temperature of 26- 28℃ under artificial light of 1500 Ix for 11 hours each day.45 days after,there were several tiny green spots appeared on the surface of some eani, which formed some somatic embryos and regenerated the entire green plantlets with their own root systems on strengthening and rooting media.