多西他赛诱导乳腺癌Bcap37细胞凋亡作用的研究

Apoptosis-inducing effect of docetaxel on the breast cancer cell line Bcap37

目的:探讨多西他赛(≤10μmol/L)体外对人乳腺癌Bcap37细胞凋亡的作用及其机制。方法:采用琼脂糖凝胶电泳观察Bcap37细胞凋亡情况;原位末端标记(TUNEL)法测定Bcap37细胞凋亡指数(apoptoticindex,AI);流式细胞仪检测Bcap37细胞周期。结果:与对照组相比,实验组琼脂糖凝胶电泳显示凋亡特有的梯状图谱;实验组显示细胞核染成棕色的凋亡细胞,且AI较对照组显著增大,具有量效依赖性,P<0·001。实验组G2/M期细胞比例较对照组显著升高,G1、S期细胞比例显著降低,P<0·01。结论:多西他赛(≤10μmol/L)诱导人乳腺癌Bcap37细胞凋亡,细胞周期阻滞在G2/M期。

OBJECTIVE： To investigate the effect of docetaxel （≤10 μmol/L） on the apoptosis of breast cancer cell line Bcap37 in vitro and elucidate its possible mechanisms. METHODS： The apoptosis of breast cancer cell line Bcap37 was observed by agarose gel electrophoresis, the cell apoptotic index （AI） was evaluated by TUNEL and the cell cycle was detected by flow cytometry. RESULTS： Compared with the control group, DNA ladder was obtained with the agarose gel electrophoresis; apoptotic cell were dyed brown in cell nuclear and AI were increased and showed a dose-dependent effect, P〈0.001; the percent of G2/M-phase cell was elevated and the percent of G1-phase and S-phase cell were lower in docetaxel groups, P〈0.01. CONCLUSION： Docetaxel （≤10 μmol/L） might induce the apoptosis in breast cancer cell line Bcap37. Its mechanism probably was due to an arrest at G2/M-phases.