高压氧对大鼠肢体缺血再灌注所致肾损伤的作用

The effect of hyperbaric oxygen on kidney dysfunction caused by rat hind limb ischemia/reperfusion

目的探讨高压氧(HBO)对减轻大鼠肢体缺血再灌注(I／R)致肾脏损伤的作用及机制。方法复制I2h／R1h模型,分为3组,即A组(假手术组)、B组(L／R模型组)、C组(HBO治疗组)。观察肾脏微血流变化;对肾脏组织行电镜病理检查;记录血清CD31、CD61平均荧光强度和CD62p的阳性率。结果与B组比较,C组肾脏微血流速度明显升高,差异有统计学意义著(P<0．05)。与B组比,C组肾脏组织病理改变较轻。与A组比较,B组CD31、CD61及CD62p均有升高,其中CD31和CD62p升高显著(P<0．05);与B组比较,C组CD31、CD61及CD62p均有下降,其中CD31和CD62p下降尤为显著(P<0．05)。结论早期行HBO处理可显著减少急性下肢L／R大鼠模型血小板膜糖蛋白CD31和CD62p的表达,从而有助于减轻下肢I／R继发肾脏损伤。

Objective To explore the potential protective effect of HBO on kidney injury caused by hind limb ischemia/reperfusion in the rat model, and to study the mechanism. Methods Adult male SD rats weighing 220～250 g were used in the study. Bilateral hind limb ischemia was induced by applying an atraumatic clip at the end of abdominal aorta adjacent to the junction site of both femoral arteries. At the end of the ischemic period, reperfusion of the limbs was achieved by releasing the clamp. The experimental animals were randomly divided into three groups, with 10 rats in each group： sham surgical group, ischemia/reperfusion （L/R） group and hyperbaric oxygen （HBO） group. By using laser Doppler flowmetry, the microvascular blood flow was measured in the kidney of the animals. Ultrastructural histological pathological changes in kidney were studied. The mean immunofluorescenee intensity of CD31, CD61 or positive percentage of CD62p were measured by the flow cytometry. Results Compared with the L/R group, the microvascular blood flow of the kidney in the HBO group was significantly increased （P 〈 0.05）. In the L/R group, extensive ultrastructural histological pathological injury of kidney occurred. The ultrastructural histological pathological change in the HBO group was obviously alleviated. Compared with the sham surgical group, the expression of platelet membrane glycoprotein CD31, CD62p in the I/R group was much higher （P 〈 0.05）. The expression of CD31, CD62p in the HBO group was much decreased than those in the I/R group （P 〈 0.05）. Conclusions As a kind of effective therapy, HBO can improve the kidney dysfunction caused by hind limb ischemia/reperfusion in rats.