复方积雪草煎剂对单侧输尿管结扎小鼠肾小管间质骨架蛋白α-SMA、vimentin表达的影响

Effects of centalla asiatica apozem compound on expression of α-SMA and vimentin in mice kedney with unilateral ureteral obstruction

目的:通过对复方积雪草煎剂对单侧输尿管结扎(unilateral ureteral occlusion,UUO)小鼠模型肾小管间质骨架蛋白α-平滑肌肌动蛋白(α-smooth muscleactin,α-SMA)、波形蛋白(vimentin)表达影响的研究,探讨其防治肾间质纤维化的作用和机理。方法:采用小鼠UUO模型,用复方积雪草水煎剂进行灌胃干预,血管紧张素转化酶抑制剂(angiotensin-convertingenzyme inhibitor,ACEI)福辛普利作为对照。MASSON染色观察肾组织病理及间质纤维化;肾组织中α-SMA、vimentin表达采用免疫组化和Western-blotting方法检测;基因表达采用逆转录-聚合酶链式反应(RT-PCR)方法。结果:UUO模型组和治疗组小鼠肾间质纤维化程度以及α-SMA、vimentin蛋白表达和基因表达水平均较假手术组显著增高,而复方积雪草煎剂、福辛普利治疗组肾间质纤维化和间质骨架蛋白的表达均较模型组显著降低。结论:复方积雪草可显著抑制肾小管上皮细胞或间质细胞的表型转化,提示降低肌成纤维细胞的积聚是其防治肾间质纤维化的重要机制之一。

AIM： To investigate the effect of centalla asiatica compound on the expression of α-smooth muscle actin （a-SMA） and vimentin in mice kedney with the unilateral ureteral obstruction （UUO）. METHODS： Mice were randomly assigned to sham operation group （sham group）, UUO model group, centella asiatica compound treatment group and ACEI treatment group. The protein expression of α-SMA and vimentin was assessed by immunohistochemistry and Western-blotting; The mRNA was detected by using reverse transcription-polymerase chain reaction analyses （RT-PCR）; And the change of renal pathology was observed at the same time. RESULTS： In comparison with the sham group, the mRNA expression and protein levels of α-SMA and vimentin in the UUO model group and treatments groups increased significantly, in addition, compared with UUO model group, centella asiatica compound and ACEI treatments could significantly ameliorate renal interstitial fibrosis and reduce the expression of α-SMA and vimentin protein. The effects of centella asiatica compound on vimentin were more significant than ACEI. CONCLUSION： Centella asiatica compound could reduce the transdifferentiation of renal tubular epithelial cells or renal interstitial cells and prevent renal interstitial fibrosis.