雄黄诱导哮喘豚鼠肺泡灌洗液中嗜酸细胞凋亡的研究

Study of realgar induced apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs

目的:观察雄黄诱导哮喘豚鼠肺泡灌洗液中嗜酸细胞凋亡,探讨雄黄治疗支气管哮喘的机理。方法:经雄黄处理后的哮喘豚鼠肺泡灌洗液中嗜酸细胞为研究对象,嗜酸细胞凋亡的形态学观察采用Giemsa染色法和电子显微镜。流式细胞仪检测嗜酸细胞凋亡率。结果:各组嗜酸细胞经雄黄处理6 h后,光镜和电镜均观察到嗜酸细胞出现凋亡的特征性改变。流式细胞仪检测显示嗜酸细胞凋亡率随着雄黄浓度的增加和与雄黄作用时间的延长而升高。结论:雄黄促进哮喘豚鼠肺泡灌洗液中嗜酸细胞凋亡,是其治疗支气管哮喘的机制之一。

AIM： To observe the realgar induced the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs and investigate the mechanism that realgar treated asthma. METHODS： The morphology of apoptosis of eosinophils was observed by Giemsa staining and electron microscope. The rate of apoptosis of eosinophils was assayed by the flow cytometry. RESULTS： The characteristic changes of the apoptosis in both light microscope and electron after 6 hours treatment of realgar microscope were shown Flow cytometry showed that the rote of apoptosis of the eosinophils was increased both increasing realgar concentration and prolonging realgar action time to the cells. CONCLUSION： Realgar promotes the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pig. Realgar induced the apoptosis of eosinophils is one of the causeses for asthmatic treatment.