保护胰岛移植物的基因治疗策略

The Strategy of Gene Therapy to Protect Islet Graft

胰岛移植是一种有望治愈糖尿病的方法,但胰岛进入体内后,由于排斥反应和其他刺激因素而使大量胰岛遭破坏,因此,减少胰岛破坏对保证移植成功非常重要。作为细胞移植物的胰岛非常适合在体外进行基因修饰,移植前对胰岛进行基因治疗以减轻局部免疫反应或增强胰岛细胞抗凋亡能力是一种极具吸引力的方法。基因转移载体及靶基因的选择是基因治疗中的关键策略。

Islet transplantation represents a potemtial curative treatment for diabetes mellitus, but considerable transplanted islets are injured by rejection and other stimulating factors after transplantation. Therefore, reduction of damage of transplanted islets may be critical for successful islet transplantation. Pancreatic islets, as a cellular graft, are especially suited for reconstitution ex vivo, and an attractive strategy to protect islets is to use gene therapy to transduce islets prior to translamation with factors that can inhibit the local immune attack or make islet cells more resistant to apoptosis. Gone transfer vectors and targeted gene are key strategies of gene therapy.