合欢种子硬实与萌发特性及种皮微形态与结构特征的研究

BOURGEON CHARACTERISTICS OF HARDSEED OF ALBIZIA JULIBRISSIN DURAZZ AND ITS TESTA MICRO-MORPHOLOGY AND STRUCTURE

光暗交替(8h/16h)、全光照、全黑暗及70,85,100℃热水浸种处理合欢硬实种子,结合扫描电镜观察和石蜡切片技术,探讨合欢硬实种子萌发特性与种皮微形态及结构特征的关系,为合欢种苗的规模生产提供理论依据。结果表明,合欢种皮对种子的吸胀作用具有明显的机械障碍作用;交替光照和70℃热水浸泡处理效果最好,发芽率达48.9%;引起合欢种皮不透性的部位可能与种皮表层、栅栏层、骨状石细胞层均有关系,合欢种子硬实现象可能与种皮无深度裂隙有关;合欢种皮侧缘厚壁(薄壁)细胞层中有维管束,它为种皮的发育提供水分与营养。

Some seeds of the Albizia julibissin Durazz were treated by illumination/dark （8h/16h）, full illumination, full darkness respectively; and simultaneously soaked in hot water under respective temperature of 70℃, 85℃, 100℃, linking with observation under SEM and technique of paraffin section, to explore their hardiness and bourgeoning characteristics, and their relation with its testa micromorphology and structure. We sought to provide theoretical basis for the scale production of the Albizia julibissin Durazz seedlings. The results showed that the testa have obvious mechanical obstacle functions to the seed imbibitions and the best results were obtained in trial when the illumination conditions were at illumination/darkness （8h/16h） and the hard -seeds were soaked in hot water under 70℃, and the germination percentage were recorded at 48.9%. The position resulting in non - permeability of its testa is likely to have something to do with surface layer, palisade layer and osteosclereid layer, and The hardiness of the seeds possibly relates to whether or not there are deep crannies in its testa. The vascular bundles in stereid layer or Parechymatous cell layer of lateral margin of its testa provide the testa development with water and nutrition.