GMP法玻璃化冷冻人胚胎的效果观察

The effect of vitrification of early human embryos by glass micropipette.

目的探索GMP法玻璃化冷人胚胎的可行性。方法选用IVF中多精受精的4-10细胞阶段的胚胎为研究对象，先后在玻璃化1液（10％乙二醇＋10％二甲亚砜）和玻璃化2液（20％乙二醇＋20％二甲亚砜＋0．3M蔗糖）中分别平衡1mim和0．5mim，然后用GMP管虹吸胚胎直接浸入液氮。其结果与常规的程序化慢冻进行比较。结果GMP法的存活率、胚胎完整率、卵裂球存活率比程序化慢冻法更高（分别为94．4％对73．9％，P〈0．01；79．6％对33．9％，P〈0．01；91．6％对67．8％；P〈0．01），但卵裂球完全死亡率更低（0％对10、4％；P〈0．01）。结论GMP法玻璃化冷冻人胚胎是可行的。

Objective： To examine the feasibility of vitrification of human 4 - 10 cell stage embryos by glass micropipette. Methods： In study group, the polyspermy embryos were pre-equilibrated in 10% EG （ethylene glycol ） ＋ 10% DMSO （dimetbyl sulfoxide） solution for 1 min, and equilibrated in the vitrification solution （20% EG ＋ 20% DMSO ＋ 0. 3 Molar/L sucrose） for 30 s, and then loaded into the glass micropipette （GMP） and frozen directly in liquid nitrogen. This results were compared with the results from programmed slow protocol. Results ： The survival rate, intact rate, total blastomere survival rate, were all significantly higher using the GMP vitrification method than using the programmed slow method （94.4% vs 73.9% , P 〈 0. 01 ; 79. 6% vs 33.9% , P 〈 0.01 ; 91.6% vs 67.8% ; P 〈0.01 ）, but the rate of the embryos that all blastomere died were just on the contrary （0% vs 10. 4% ; P 〈 0.01）. Conclusion： Early human embryos can be successfully vitrified by the simple GMP method.