摘要
目的检测胎盘组织中TGF-β1(transform ing growth factor-beta1,TGF-β1)及其受体TGFβRⅡ(TGF-beta receptorⅡ)的表达,探求TGF-β1在子痫前期发病中的作用。方法用半定量逆转录聚合酶链反应(RT-PCR)技术对10例正常妊娠组和26例子痫前期组(包括12例轻度和14例重度)胎盘组织中TGF-β1、TGFβRⅡmRNA的表达水平进行检测,并通过紫外凝胶图像分析进行定量分析。结果重度子痫前期组TGF-β1和TGFβRⅡmRNA的表达量均升高,与轻度子痫前期组及正常妊娠组相比有明显意义(P<0.05)。结论TGF-β1、TGFβRⅡ在转录水平就已经上调,它们的异常表达它们可能与子痫前期的发病有着重大的关系。
Objective： To detect the mRNA expression of transforming growth factorbetα1 （TGF- β1 ） and its receptor （TGF β RⅡ） in placentas in order to evaluate the role of TGF - β1 in preeclampsia. Methods ： The semi - quantitative method of reverse transcription polymerase chain reaction （RT- PCR） was employed to investigate the mRNA expression levels of TGF - β1 , TGF β RⅡ in placenta tissue of 10 cases of normal term pregnant women and 26 cases of women with preeclmpsia （ 12 cases of mild preeclampsia and 14 cases of severe preeclampsia）, the mean density was determined by image analysis technique. Results： Compared with the mild preeclampsia group and the normal group, the mRNA expression levels of TGF -β and TGF β RⅡ of severe preeclampsia group was higher （ P 〈0. 05）. Conclusion The expression of TGF β1 and TGF β RⅡ was already changed at the level of transcription, the abnormal expression of TGF - β1and TGF Ⅱ RⅡ maybe play an important role in the pathogenic causes of preeclampsia.
出处
《中国优生与遗传杂志》
2006年第11期14-15,13,共3页
Chinese Journal of Birth Health & Heredity
