早期先兆流产孕妇HCMV感染与血清PAPP-A水平的相关性研究

The correlation between active infection of HCMV and the serum level of PAPP-A in women with threatened abortion in early trimester of pregnancy.

目的探讨早孕妇女妊娠相关血浆蛋白A(PAPP-A)水平与人巨细胞病毒(HCMV)活动性感染的相关性以及二者在早期自然流产发病机制中的作用。方法应用酶联免疫吸附试验(ELISA)和逆转录PCR(RT-PCR)筛选HCMV活动性感染早孕先兆流产妇女,定量检测其外周血血清PAPP-A水平,并与相同孕周的非HCMV活动性感染的先兆流产孕妇及健康孕妇进行比较分析。结果早期先兆流产妇女中HCMV活动性感染者、非活动性感染者、健康孕妇血清PAPP-A分别为:(0.4924±0.088)m lU/m l,(0.805±0.213)m lU/m l和(2.479±1.020)m IU/m l(F=93.98,P<0.01)。结论早孕妇女HCMV活动性感染和PAPP-A水平密切相关,并可能在早期自然流产的发病机制中占据相当重要的地位。

Objeetive： To probe into the correlation between active infection of human cytomegalovirus （HCMV） and the serum level of pregnancy - associated plasma protein （ PAPP - A） in early trimester of pregnancy and their role in the pathogenesis of spontaneous abortion. Methods： HCMV IgM and mRNA in the sera of women with threatened abortion in early trimester of pregnancy were tested with enzyme - linked immunosorbant assay （ ELISA ） and reverse transcription polymerasechain reaction （ RT - PCR ） and those with active infection of HCMV were screened. Non - infected gravida of threatened abortion and healthy gravida of similar gestational age were randomly selected. The serum level of PAPP - A of the three groups was assayed with ELISA. Results： The serum level of PAPP - A was （0. 4924 ± 0. 088） mlU/ml, （0. 805 ±0. 213 ） mtU/ml and （2. 479 ± 1. 020 ） mlU/ml, respectively （ F =93.98, P 〈0. 01 ）. Conclusion： The serum PAPP- A is correlated with active infection of HCMV, which might play an essential role in the pathogenesis of spontaneous abortion in early trimester of pregnancy.