快速筛选金钱松内生真菌抗真菌活性的研究

Study on fast screening antifungus activity of endophytes from Pseudolarix kaempferi

目的:筛选金钱松内生真菌抗病源真菌的活性菌株。方法:首次对金钱松内生真菌进行了分离,以稻瘟霉PyricuLaria oryzaP-2b为筛选模型,采用微量稀释法筛选金钱松内生真菌抗真菌活性,研究其抗病源真菌的能力。结果:金钱松内存在着大量且具有抗稻瘟霉P-2b活性的内生真菌,占内生真菌总数的44.8%。其中JJ314,JJ323可诱导稻瘟霉P-2b菌丝念珠状、膨大变形,JJ324可抑制孢子萌发,它们对红色毛癣菌Trichophyton rubrum、新型隐球菌Cryptococcus neoformans、白色念珠菌Candida albicans等病源真菌都具有强烈的活性。结论:金钱松内生真菌是潜在的抗真菌药物资源。

Objective： To screen antifungal activity of endophytes from Pseudolarix kaempferi. Method： Endophytes from P. kaempferi were separated. By means of microdilution method, antifungal active endophytes were fast screened by Pyricuiaria oryzae P- 2b model, and activity of endophytes against pathogenic fungus was studied. Result： 44. 8% of endophytes showed activity againstP. oryzae P-2b in Pseudolarix kaempferi. Among them JJ314, JJ323 introduced formation of characteristic beads and swellings on the growing hyphae, JJ324 inhibited the conidia germination. They all showed activity against Trichophyton rubrum, Cryptococcus neoformans and Candida albicans. Conclusion： Endophytes from P. kaempferi are a potential resource for the development of antifungal agent.